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An alloantiserum was prepared in C57BL/10 mice hyperimmunized with concanavalin A-stimulated B10.HTG thymocytes. Absorption with separated B10.A(3R)B and T lymphocytes, followed by titration on these cell types, revealed a new I-region controlled T-cell specificity, Iat.46. The determinant is present on a subpopulation of mature peripheral T cells but not on B lymphocytes, thymocytes, or bone marrow cells. It is also observed using (A.TH X B10.HTT)F1 anti-A.TL antiserum on B10.A(3R) T lymphocytes. The k and d haplotypes are fully crossreactive with regard to Iat.46 expression. B10.HTT, C57BL/10, and BSVS do not carry the Iat.46-encoding allele. Moreover, Iat.46 is not a determinant formed by I-A--I-E gene complementation. The Iat.46-controlling gene, Ia-8, may be positioned to the left of the B10.HTT crossover and to the right of the B10.A(3R) recombinational event. Thus, this determinant may be considered either to represent complexity within the I-J subregion or to demarcate a new I subregion between I-J and I-E.

Cytoplasmic and nuclear receptor sites of the prostate will be determined for a qualitative information about the pattern of specific bindings. Their qualitative evaluation and their selective suppression will give an idea about the hormonal influence and regulation in prostatic tissue. These investigations are performed in some cases of prostatic cancer. The difficulty of tissue preparation is caused by the individual distribution of collagen tissue in prostatic cancer which will decide the yield of purified receptor sites. Besides the elemination of unspecific binding sites like SHBG and albumin is an evidence for the value of a method. According to high amount of binding sites the most effective preparation of patients before a tissue biopsy is discussed. For the clinical application the DCC test in case of cytoplasmic sites and the exchange test for nuclear sites seem to provide a suitable method. Performing gelfiltration and additional test with denatured material is necessary because of the similar size of SHBG and receptor complexes interfering the results.